If the VTG jumper is removed, VTG must be supplied from an external source.
If you are using one of the MCU boards mounted on an STK600, the STK600 can supply the power. Consult the STK600 user guide on how to set the voltage.
If you are using the MCU board stand-alone, you must connect a power supply to the MCU board. Connect the source to any of the VTG/GND pins on either QT600 or the MCU board.
If you are using a custom MCU board, you have to supply the right voltage yourself.