To program the device on the STK505 using the In-System Programming (ISP) mode, connect a 6-pin cable between the ISP6PIN connector on STK500 and the SPROG connector on STK505 colored section corresponding to the device used. I.e. if programming ATtiny24, connect the 6-pin cable to the SPROG header on the green section, as shown in Figure 1. If programming ATtiny26 connect the 6-pin cable to the SPROG header on the blue section, as shown in Figure 2. Then start the Programming Dialog in AVR Studio
To be able to ISP program the AVR, the ISP programming enable fuse must be set. The ISP programming enable fuse can be programmed by High Voltage Programming.